All DNA samples sent must be provided at a concentration of 50-100ng/ul,
1 ug total (~20 ul @ 50ng/ul). Concentrations should be determined with
a fluorimeter using Pico-green. Quant-iT PicoGreen dsDNA Assay can be
purchased through Invitrogen.
DNA Plate Layout
We require that only 92 DNA samples should be plated in a 96 well v-bottom plate; the remaining four wells ( H9-H12) on each plate are reserved for controls. Plates should be uniquely labeled with PI name, date, and plate number, for example, johnsmith_83005_plate_1.xls. A DNA manifest in hard copy form should accompany your DNA samples. In addition, an electronic version should be sent to firstname.lastname@example.org. Save each DNA plate layout in a separate file. Use the format template below as a guide for the DNA manifest. Although we don't require ethnicity and gender, it is useful for validation purposes.
Shipment of DNA
Samples should be shipped by FedEx Overnight Express. Please package the samples
on dry ice in a Styrofoam container. The weight and amount of dry ice will
be recorded on the container. Please email the shipping tracking number to